Depending upon the chemical composition of the analyte, the molecules are retarded while in the column stationary phase as a consequence of specific intermolecular interactions in between the analyte plus the packing product around the column.Notice: If you are interested, You will find there's complete area about UV-visible spectroscopy on the i
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In the separation, the molecules jogging from the column can also be considered as becoming inside a constant equilibrium between the cell section and also the stationary period. This equilibrium may be governed by an equilibrium constant K, outlined as ref eight , by which Cmo will be the molar concentration in the molecules from the cell stage, a